Synonym(s): FGF2; bFGF
CAS No.: 106096-93-9
Purity: ≥97% (SDS-PAGE)
Application: FGF-2 is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines such as mesenchymal stem (stroma) cells (MSC).
General description: FGF2 is found in basement membranes and sub-endothelial extracellular matrix. FGF2 is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF2 is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF2 in human ES cells.
Physical form: Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note: The bioactivity of bFGF was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
Storage: −20°C
Empirical Formula (Hill Notation): C6H5FO