ExtrAvidin(R)-Peroxidase, buffered aqueous solution

MDL No.: MFCD00163600

Storage: 2-8C

UNSPSC Code: 12352203

Application: ExtrAvidin(R)-Peroxidase is suitable for the following applications: . Dot blot at a minimum dilution of 1:4,000 . Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100). Direct ELISA at a minimum dilution of 1:2,000It was used for . both Immunoprecipitation and Immunobloting. Ra2I solid-phase binding assays. Southwestern blotting . Western blotting. Immunocytochemistry

Biochem/physiol Actions: Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin(R) is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Preparation Note: Affinity purified protein