Application: EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic® F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information: EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
Storage: 2-8°C
EC No.: 231-791-2
Empirical Formula (Hill Notation): C63H88CoN14O14P