Synonyms: N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride
CAS Number: 132172-61-3
Formula: C42H80ClNO4
Formula Weight: 698.54
Linear Formula: C42H80ClNO4
MDL No.: MFCD01310872
Purity: >=98% (TLC)
Storage: -20C
UNSPSC Code: 12352200
Application: Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 mug DOTAP per mug DNA and approximately 10-20 mug DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
RIDADR: NONH for all modes of transport
WGK Germany: 3