Synonyms: Polydeoxyribonucleotide Synthase; Polynucleotide Ligase; T4 DNA Ligase
CAS Number: 9015-85-4
MDL No.: MFCD00163508
Activity: activity: 4,000 U/mL
Storage: -20C
Enzyme Commission (EC) Number: 6.5.1.1 ( BRENDA | IUBMB )
EC Number: 232-770-0
UNSPSC Code: 12352204
Application: Suitable for:. Ligation of blunt ended or cohesive DNA fragments. Ligation of cloning vector and restriction insert fragments. Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids. Couple RNA single strands by bridging oligonucleotide adapters
Biochem/physiol Actions: T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.
Components: T4 DNA Ligase is supplied in a solution containing 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.
Unit Definition: One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37C.
Other Notes: T4 DNA Ligase is inactivated by heating at 65 C for 10 minutes.
RIDADR: NONH for all modes of transport
WGK Germany: 3