MDL No.: MFCD00211877
Storage: -70C
Enzyme Commission (EC) Number: 5.99.1.3 ( BRENDA | IUBMB )
UNSPSC Code: 12352204
Application: DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.
Biochem/physiol Actions: Can be used to supercoil plasmids.
Biochem/physiol Actions: DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.
Unit Definition: One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 C.
Other Notes: Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.
RIDADR: NONH for all modes of transport
WGK Germany: 1