SIGMA DL-Dithiothreitol, for molecular biology, =98% (TLC), =99% (titration)

Synonyms: threo-1,4-Dimercapto-2,3-butanediol; Cleland's reagent; DTT

Formula: HSCH₂CH(OH)CH(OH)CH₂SH

Formula Weight: 154.25

CAS No.: 3483-12-3

Purity: ≥98% (TLC); ≥99% (titration)

Storage Temperature: 2-8°C

Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).