Chondroitinase AC from Flavobacterium heparinum, recombinant, expressed in E. coli, >=200 units/mg protein, For Chondroitin Sulfate Analysis

Synonyms: Chondroitin AC lyase

CAS Number: 9047-57-8

MDL No.: MFCD00130796

Purity: >=90% (SDS-PAGE)

Storage: -20C

Enzyme Commission (EC) Number: 4.2.2.5  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Unit Definition: 1 unit is defined as the amount of enzyme that will liberate 1.0 mumole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 C as measured by the change in A232. The epsilonmuΜ for the reaction product Delta-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Delta-Di-6S (chondroitin sulfate C).

Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer