SIGMA Chloramphenicol, meets USP testing specifications

Synonyms: D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide; D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol; D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide; Chloromycetin

Formula: Cl₂CHCONHCH(CH₂OH)CH(OH)C₆H₄NO₂

Formula Weight: 323.13

CAS No.: 56-75-7

Application: Used as a seletion agent for transformed cells containing chloramphenicol resistance genes. Recommended for use in cell culture applications at 10 ml/L.

Biochem/physiol Actions: Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.
Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).