Synonym(s): H2O2:H2O2 oxidoreductase
CAS No.: 9001-05-2
Application: Catalase is used to eliminate H2O2 from enzymatic oxidation reactions.1,
Physical properties: Catalase from bovine liver is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group.
Analysis Note: Protein determined by biuret.
Storage: −20°C
EC No.: 232-577-1
Biochem/physiol Actions: Activates the decomposition of hydrogen peroxide, Natural antioxidant used to study roles of reactive oxygen species in gene expression and apoptosis.
Unit Definition: One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Empirical Formula (Hill Notation): C9H8N2