Synonym(s): Abrineurin; BDNF
Purity: ≥98% (SDS-PAGE and HPLC)
General description: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of recombinant human BDNF (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Physical form: The product is lyophilized from a sterile 0.2 μm filtered solution containing 250 μg BSA
Analysis Note: The bioactivity is determined by its ability to stimulate the proliferation of rat C6 cells.
Storage: −20°C
Biochem/physiol Actions: Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Empirical Formula (Hill Notation): CH6N4 · H2CO3