General description: Biglycan is a 200-350 kDa(proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains. Biglycan binds to TGF-+ƒ. It also binds to collagen type I in low ionic strength (less than 3 mM phosphate) buffer. At higher ionic strengths, biglycan does not bind to collagen type I. It enhances the inhibition effect of TGF-+ƒ on osteoclast proliferation at a concentration of 4-20 mg/ml. It also prevents the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/ml.
Storage: −20°C
Biochem/physiol Actions: Biglycan is a leucine-rich repeat proteoglycan that interacts strongly with collagen types I and II. Biglycan is an extracellular component that binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.
Empirical Formula (Hill Notation): C2H5ClO