BID, Caspase-8-cleaved from mouse, >=95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution

MDL No.: MFCD03456214

Purity: >=95% (SDS-PAGE)

Storage: -20C

UNSPSC Code: 12352202

General description: Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.

Biochem/physiol Actions: Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.

Physical form: 0.2 mum filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.

Analysis Note: Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.