Synonyms: beta-D-Galactoside galactohydrolase; Lactase
CAS Number: 2604851
MDL No.: MFCD00130623
Storage: -20C
Enzyme Commission (EC) Number: 3.2.1.23 ( BRENDA | IUBMB )
EC Number: 232-864-1
UNSPSC Code: 12352204
Application: Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Biochem/physiol Actions: β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical properties: Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Unit Definition: 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 C
Other Notes: Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590); Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique; Enzyme immunoassay of thyrptropin releasing hormone (TRH); Preparation of enzyme-antibody conjugates
RTECS: LW5840000
RIDADR: NONH for all modes of transport
WGK Germany: 3