MDL No.: MFCD00130585
Storage: -20C
UNSPSC Code: 12352203
General description: Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Application: Avidin-FITC from egg white can be used for immunohistochemistry in formalin-fixed, paraffin-embedded sections at a concentration of 20-40 mug/mL. The product can also be used for indirect immunofluorescence (1:200).
Application: To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.
Physical form: Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.
Preparation Note: Affinity purified protein.
RIDADR: NONH for all modes of transport
WGK Germany: nwg