Synonyms: Adenosine 5'-diphosphatase; Adenosine 5'-triphosphatase
CAS Number: 9000-95-7
MDL No.: MFCD00130542
Activity: secondary activity: >=50 % of base activity ADPase
Storage: -20C
Enzyme Commission (EC) Number: 3.6.1.5 ( BRENDA | IUBMB )
UNSPSC Code: 12352204
Application: At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).Reaction: ATP → ADP+Pi → AMP+2Pi.
Application: Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.
Biochem/physiol Actions: Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Physical form: Lyophilized powder containing potassium succinate buffer salts.
Unit Definition: One unit will liberate 1.0 mumole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 C.
Preparation Note: Derived from red potato
RIDADR: NONH for all modes of transport
WGK Germany: 3