Anti-PMP70 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-70 kDa Peroxisomal membrane Protein; Anti-ABCD3; Anti-PXMP1

MDL No.: MFCD01865555

Storage: -20C

UNSPSC Code: 12352203

General description: Rabbit polyclonal anti-PMP70 antibody recognizes rat, mouse, and human 70 kDa Peroxisomal membrane Protein (PMP70). Detection of the PMP70 band by immunoblotting is specifically inhibited with the immunizing peptide.

General description: The 70 kDa peroxisomal membrane protein (PMP70, PXMP1, ABCD3, ABD3) is a major component of peroxisomal membranes. The peroxisome is a multifunctional single-membrane organelle present in nearly all eukaryotic cells. One of the most important metabolic processes of the peroxisome is the beta-oxidation of long and very long chain fatty acids. Peroxisomes are also involved in detoxification of the cell by the enzyme catalase that decomposes hydrogen peroxide, a toxic byproduct of cellular metabolism. PMP70 belongs to the ALD subfamily of the ATPbinding cassette (ABC) transporter superfamily. PMP70 participates in the metabolic transport of long and very long fatty acids into peroxisomes. It forms a stable complex with the adrenoleukodystrophy protein, ALDP, and several other peroxisomal proteins. ATP binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)

Application: Rabbit polyclonal anti-PMP70 antibody is used to tag 70 kDa peroxisomal membrane protein for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting (70 kDa), immunoprecipitation and immunofluorescence. It is used as a probe to determine the presence and roles of 70 kDa peroxisomal membrane protein in the proliferation and function of peroxiosomes. Anti-PMP70 may useful in studies of Zellweger syndrome-2.

Immunogen: synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.