Synonyms: Anti-phospho-EGFR (pTyr1068)
MDL No.: MFCD03845325
Storage: -20C
UNSPSC Code: 51111800
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General description: The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R is present on many cell types of epithelial and mesenchymal lineages.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)
Application: Anti-phospho-EGFR [pTyr1068] antibody may be used at a working dilution of 1:1000 for immunoblotting using NIH3T3 cells expressing human EGF R, stimulated with EGF. The antibody has been in detection of EGF R [pTyr1068] in human lymphocytes.
Biochem/physiol Actions: The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is capable of binding transforming growth factor-a and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Phosphorylation of Tyr1068 occurs by autophosphorylation of EGF R and is required for the binding of Grb2 and activation of ERK1/2 pathway Anti-phospho-EGFR [pTyr1068] specifically recognizes human and rat EGFR phosphorylated at tyrosine 1068.
Immunogen: synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 1068.
Physical form: Solution in Dulbecco's phosphate buffered saline, with 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3