MDL No.: MFCD08705496
Storage: -20C
UNSPSC Code: 12352203
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General description: DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.
Application: Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.
Biochem/physiol Actions: Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac(R).
Immunogen: synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.
Physical form: Solution at 100 muL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol.
Target description: DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA.
Other Notes: The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment.
RIDADR: NONH for all modes of transport
WGK Germany: 2