Anti-Mouse IgG (whole molecule) F(ab')2 fragment-Cy3 antibody produced in sheep, affinity isolated antibody, buffered aqueous solution

MDL No.: MFCD02179333

Storage: 2-8C

UNSPSC Code: 12352203

Application: Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.

Application: The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

Biochem/physiol Actions: IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note: Adsorbed to reduce background staining with human samples.

Other Notes: Antibody adsorbed with human serum proteins.