MDL No.: MFCD00162623
Storage: -20C
UNSPSC Code: 12352203
General description: Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.Goat anti-mouse IgG (Fab specific)-peroxidase antibody associates with mouse IgG and mouse Fab fragment. The conjugate shows no reactivity with mouse Fc fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.
General description: Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense and is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Application: Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in western blotting.
Application: Goat anti-mouse IgG (Fab specific)-peroxidase antibody is suitable for use in immunohistochemistry applications at 1:200 dilution using human tonsil tissues. The antibody can also be used for western blot assays at 1:80,000-1:160,000 dilutions using total cell extract of HeLa cells.
Application: Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal.
Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Preparation Note: Adsorbed to reduce background staining with bovine, horse and human samples.
Other Notes: Antibody adsorbed with bovine, equine, and human serum proteins.
RIDADR: NONH for all modes of transport
WGK Germany: 3