MDL No.: MFCD00162620
Storage: 2-8C
UNSPSC Code: 12352203
General description: Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.
Application: Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody can be used in western blot analysis using E. coli lysates . The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (1:25 using formalin-fixed, paraffin-embedded sections).
Application: Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Immunogen: Purified mouse IgG, Fab fragment
Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Preparation Note: Adsorbed to reduce background staining with bovine, horse, or human samples.
Other Notes: Antibody adsorbed with bovine, equine and human serum proteins.
RIDADR: NONH for all modes of transport
WGK Germany: 2