MDL No.: MFCD03455383
Storage: -20C
UNSPSC Code: 12352203
General description: Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins.Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin.
Application: Rabbit anti-l/s-Afadin antibody has been used for immunohistochemistry (frozen sections, 1:1,000 using mouse liver sections) and indirect immunofluorescence (1:250 using cultured dog MDCK cells, and cultured human HepG2 cells) techniques. It can also be used for immunocytochemistry, microarray and western blot (1:1000 using rat brain extracts) assays.
Immunogen: synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
RIDADR: NONH for all modes of transport
WGK Germany: 2