Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-FGFR-2

MDL No.: MFCD02096345

Storage: -20C

UNSPSC Code: 51111800

Features and Benefits: Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description: Fibroblast growth factors (FGFs) are members of a large tyrosine kinase family of growth factor receptors (17-38kDa) that are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted8 or possibly cleaved9 forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid 'acidic box', a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

General description: Fibroblast growth factors (FGFs) are members of a large tyrosine kinase family of growth factor receptors (17-38kDa) that are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted8 or possibly cleaved9 forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Application: Immunoblotting: a minimum working antibody dilution of 1:2,000 is determined using a whole extract of transfected cells expressing recombinant human FGFR-2. Immunohistochemistry: a minimum working dilution of 1:1,000 is determined using indirect immunoperoxidase staining and trypsin-digested, formalin-fixed, paraffinembedded human and animal tissue sections.

Immunogen: synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity: The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

Specificity: The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.

Preparation Note: Affinity isolated on a peptide-agarose column.