Synonyms: Anti-Eukaryotic Release Factor 3a; Anti-G1 to S phase transition 1; Anti-GST1, homolog of yeast
MDL No.: MFCD09265357
Storage: -20C
UNSPSC Code: 12352203
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Application: Anti-eRF3a/GSPT1 antibody produced in rabbit is suitable for the following applications:. Immunoprecipitation at a concentration of 1-2mug using HeLa cell lysates. Indirect immunofluorescence at a dilution of 5-10mug/mL using paraformadehyde-fixed HeLa cells. Western blotting at a concentration of 0.5-1mug/mL using HeLa cell lysates
Biochem/physiol Actions: Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1) in humans. The eRF3a/GSPT1 exon 1 contains a tri-nucleotide GGC repeat coding for a polyglycine expansion in the N-terminal of the protein. It is associated with protein translation as a regulator of apoptosis signal-regulating kinase 1 (ASK1). GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity by activating caspase-3. It plays a novel role in the regulation of ASK1-mediated apoptosis. GSPT1 is found to be up-regulated in various human cancers and is considered as a potential proto-oncogene.
Immunogen: synthetic peptide corresponding to amino acids 190-204 of human eRF3a/GSPT1, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3