Anti-EB1 antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-APC binding protein EB1; Anti-End-Binding-1; Anti-Microtuble-associated protein RP/EB family member 1

MDL No.: MFCD01633308

Storage: -20C

UNSPSC Code: 12352203

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Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunohistochemistry (1 paper)

Application: Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. Anti-EB1 antibody produced in rabbit is suitable for the following applications: . Immunoprecipitation at a concentration of 10-20 mug using cell lysate of the human epitheloid carcinoma HeLa cell line. Indirect immunofluorescence at a concentration 1-2 mug/mL using rat fibroblast Rat1 cells. Indirect immunofluorescence. Western blotting at a concentration of 0.5-1 mug/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2

Biochem/physiol Actions: Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. End-binding proteins (EBs) consist of a highly conserved family of microtubule plus end-tracking proteins. Its C-terminal domain is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

Immunogen: synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.