Anti-Drebrin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Synonyms: Anti-developmentally-regulated brain protein

MDL No.: MFCD03845304

Storage: -20C

UNSPSC Code: 12352203

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General description: Drebrin is a member of the newly identified ADF-H family of actin-binding proteins that share the structurally conserved actin-depolymerizing factor (ADF) binding module. It is composed of a single ADF-H domain at its N-terminus, followed by a non-conserved central region and a C-terminal SH3 domain. Three isoforms, E1 and E2 (embryonic) and A (adult), have been identified in the brain. An additional truncated form of drebrin A, s-drebrin/A2, is specifically expressed in the adult brain, but not in non-neuronal tissues. Drebrin is also widely expressed in a variety of cells including epithelial, endothelial, and smooth muscle cells and is associated at cell-cell adhering junction sites.

Application: Anti-Drebrin antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:1000 using a rat brain cytosol extract and a whole extract of the human epitheloid carcinoma HeLa cell line. It was used as a primary antibody at 1:1000 working dilution for western blotting of protein sample extracted from hippocampal tissues in a study.

Biochem/physiol Actions: Drebrin (developmentally-regulated brain protein) is a major neuronal F-actin binding protein involved in the control of actin dynamics and neuronal morphogenesis. It colocalizes with actin filaments and dendritic-like cell processes and may be involved in actin remodeling. It binds to and dissociates F-actin stabilizing proteins such as α-actinin, fascin, and tropomyosin from actin filaments. It plays an important role in the formation of axons and dendrites during neuronal development and in neuronal plasticity in the adult brain.

Immunogen: synthetic peptide corresponding to the N-terminal region of human drebrin E/A (amino acids 22-42) conjugated to KLH.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.