Synonyms: Anti-DNA methyltransferase
MDL No.: MFCD06201933
Storage: -20C
UNSPSC Code: 12352203
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General description: In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.
Application: Anti-DNMT1 antibody produced in rabbit is suitable for microarray, immunofluorescence at a working dilution of 1:100-1:200 using mouse COS-7 cells and western blot analysis at 1:1000-1:2000 dilution using nuclear extracts of human HEK 293T cells. Antibodies reacting specifically with DNMT1 may be useful for the study of chromatin remodeling effects on gene expression. It was used as a primary antibody at 1:2000 working dilution to detect DNMT1 protein in protein sample extracted from colon cancer cells transfected with DNMT1 vectors.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)
Biochem/physiol Actions: DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.
Immunogen: synthetic peptide corresponding to amino acids 1581-1595 of rat DNMT1, conjugated to KLH via an N-terminal added lysine residue. The sequence is conserved in human and mouse.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 2