Anti-DHFR, N-terminal antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-Dihydrofolate reductase

MDL No.: MFCD05665212

Storage: -20C

UNSPSC Code: 12352203

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General description: Dihydrofolate reductase (DHFR) is an NADPH dependent enzyme that reduces dihydrofolate and regenerates tetrahydrofolate. DHFR is involved in the biosynthesis of thymidylate and purines, and thereby regulates DNA synthesis and cell survival. Alterations in DHFR have been associated with impaired nucleic acid synthesis and cell death. Thus, this enzyme has important therapeutic implications in cell proliferative disorders such as cancer . Anti-DHFR, N-terminal antibody is specific for the epitope residing within amino acids 27-40 of mouse DHFR. The antibody interacts with DHFR and DHFR fusion proteins. The product is specific for DHFR in humans, mice and rats. Staining of the DHFR band by immunoblotting is inhibited by the immunizing peptide.

Application: Anti-DHFR, N-terminal antibody is suitable for use in chemiluminescent western blot (0.5-1.0 mug/mL using 100 ng of purified recombinant DHFR) and immunoprecipitation (0.5-1.0 mug using 100-200 ng of purified DHFR). The antibody may also be used for immunoblot assays.

Immunogen: amino acids 27-40 of mouse DHFR, conjugated to KLH via a C-terminal added cystein residue.

Immunogen: The immunizing sequence is conserved in mouse, human and rat.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.