Synonyms: Anti-Actin Depolymerizing Factor
MDL No.: MFCD03791499
Storage: -20C
UNSPSC Code: 12352203
Features and Benefits: Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description: Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in 'Hirano bodies' in certain brain neurons of dementia patients.
Application: Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent.
Biochem/physiol Actions: Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.
Immunogen: synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: nwg
R Codes: 21/22
S Codes: 36/37
IOD Codes: Xn