Synonyms: Anti-DERL3; Anti-Der1-like domain family, member 3
Storage: -20C
UNSPSC Code: 12352203
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Application: Anti-Derlin-3 antibody produced in rabbit is suitable for immunoblotting at a working concentration of 2.0-5.0 mug/mL using a whole extract of HEK-293T cells expressing recombinant human Derlin3. It is also suitable for immunofluorescence at 10-20 mug/mL using human HeLa cells and immunoprecipitation at a working amount of 5-10 mug using an extract HEK-293T cells expressing recombinant human Derlin-3.
Biochem/physiol Actions: Derlin-3 is involved in the degradation of misfolded glycoproteins in the ER. Derlin-3 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-3 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex.
Immunogen: synthetic peptide corresponding to amino acid residues 192-205 of human Derlin-3, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse and 2 amino acids in rat.
Physical form: Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport