MDL No.: MFCD00162231
Storage: -20C
UNSPSC Code: 12352203
General description: Cholera toxin, the pathogenic agent of cholera, is made of two subunits, A (27 kDa) and B (12 kDa) assembled with the stoichiometry AB5. The B-subunit binds to specific receptors, the monosialogangliosides GM1, located in the membrane of intestinal epithelial cells. The A1 fragment of the A-subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the Gsa regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells.
General description: The antiserum reacts versus Cholera toxin, but shows no reaction versus Staphylococcal enterotoxin A, Staphylococcal enterotoxin B and Pseudomonas exotoxin A (protein concentration: 50-500 ng/dot). The product has not been tested for neutralization potency against active Cholera toxin.
Application: Rabbit polyclonal anti-Cholera Toxin is used to tag cholera toxin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It may be used in studies on basic structural and functional aspects of toxin-membrane interactions.
Immunogen: toxin from Vibrio cholerae
Preparation Note: delipidized
RIDADR: NONH for all modes of transport
WGK Germany: 2
R Codes: 21/22
S Codes: 36/37-60
IOD Codes: Xn