Synonyms: Anti-Caveolin-1; Anti-VIP21
MDL No.: MFCD01633097
Storage: -20C
UNSPSC Code: 12352203
Application: Anti-Caveolin-1 antibody produced in rabbit is suitable for:. indirect immunofluorescence: 4-8mug/mL using HUVEC cell line. indirect immunofluorescenc. western blot at a concentration of 1-2mug/mL using whole cell extract of the human endothelial ECV304 cell line and the mouse fibroblast NIH3T3 cell line. detection and localization of caveolin-1 by immunoblotting (22 kDa) and immunofluorescence.Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions: Caveolin, a 20-24 kDa integral transmembrane protein, has been identified as a principal component of caveolae membranes. Anti-Caveolin-1 recognizes caveolin-1.
Immunogen: synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 (amino acids 2-20).
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Specificity: The sequence is highly conserved in many species, e.g., identical in rat, mouse, bovine, and dog caveolin-1. The sequence is not found in caveolin-2 or caveolin-3.
RIDADR: NONH for all modes of transport
WGK Germany: nwg