Anti-beta-Catenin antibody produced in rabbit, whole antiserum

MDL No.: MFCD02685970

Storage: -20C

UNSPSC Code: 12352203

General description: The distinct peripheral cytosolic proteins, alpha-, beta- and gamma-catenin (102 kDa, 94 kDa and 86 kDa respectively), are found in varying abundance in many developing and adult tissues. The catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules, concentrated at adherens junctions that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis.Plakoglobin (probably identical to gamma-catenin) and beta-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. The protein, beta-catenin, binds directly to the cytoplasmic tail of Ecadherin. It binds to the amino terminus of alpha-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene APC. The central core region of beta-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, beta-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and Wnt-1. p120cas which exhibits structural similarity to beta-catenin and plakoglobin may represent another catenin associated with cadherin.

Application: Polyclonal antibodies against defined type-specific catenin peptides are useful tools for the study of these proteins. Such antibodies recognize the respective catenin type in a variety of immunological techniques such as immunoprecipitation, immunoblotting, immunofluorescence and immunoperoxidase in different cell types from various species.

Immunogen: synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse beta-catenin, conjugated with gluteraldehyde to KLH.

Specificity: The antibody does not cross react with alpha-catenin or gamma-catenin (plakoglobin).