Anti-ATG5 (N-terminal) antibody produced in rabbit, affinity isolated antibody, PBS solution

Synonyms: ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit; Atg5 Antibody; Anti-APG5; Anti-APG5L; Anti-ASP; Anti-ATG5 autophagy related 5 homolog (S. cerevisiae); Anti-Apoptosis-specific protein; Anti-Autophagy protein 5-like

MDL No.: MFCD09753847

Storage: -20C

UNSPSC Code: 12352203

General description: Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation,. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties,. Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Application: Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Application: Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Immunogen: synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide