Anti-A1 Adenosine Receptor antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

MDL No.: MFCD02660703

Storage: -20C

UNSPSC Code: 12352203

General description: Anti-A1 Adenosine Receptor is specific for A1 adenosine receptor adenosine receptor subunit. By immunoblotting, it reacts strongly with human and rat, and weakly with bovine A1. The antibody detects A1 adenosine receptor in human hippocampus by immunohistology and may be used for immunoprecipitation and confocal microscopy

General description: Adenosine receptors (ARs) are members of the 7-transmembrane domain G protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of Ars ubiquitously mediates cell signalling pathways that protect tissues and organs from damage . Additionally, Ars modulate immune functions of the brain and alters the neurotransmission by glutamatergic and dopaminergic systems The A1AR is a glycoprotein of MW 36-40 kDa that can activate Gi and Go proteins in vitro In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of K+ channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release In white adipocytes, A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis

General description: ADORA1 is an adenosine receptor which is part of the G-protein coupled receptor family of proteins. ADORA1 functions as a receptor for adenosine to regulate vasodilation and vasoconstriction in cardiac tissue and kidneys.

Application: Rabbit anti-A1 adenosine receptor antibody can be used for immunohistochemistry and immunoblotting assays using rat tissues. The antibody can also be used for immunocytochemistry assays using rat spinal cord tissues. The product may also be used for immunoprecipitation applications.

Immunogen: synthetic peptide (Gln-Pro-Lys-Pro-Pro-Ile-Asp-Glu-Asp-Leu-Pro-Glu-Glu-Lys-Ala-Lys-Ala-Glu-Asp) derived from amino acids 309-326 of the rat A1 adenosine receptor C-terminal domain.

Physical form: Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.