Synonyms: Anti-alpha-Catenin; Alpha Catenin Antibody; Alpha Catenin Antibody - Anti-alpha-Catenin antibody produced in rabbit
Storage: -20C
UNSPSC Code: 12352203
General description: Rabbit polyclonal anti-alpha-Catenin antibody is used to detect various mammalian species alpha-catenin proteins. The antiserum show no cross-reactivity with beta-catenin peptide (amino acids 768-781) conjugated to BSA. Does not cross-react with beta-catenin or gamma-catenin (plakoglobin).
General description: The distinct peripheral cytosolic proteins, alpha-, beta- and gamma-catenin (102 kDa, 94 kDa and 86 kDa, respectively) are found in varying abundance in many developing and adult tissues. The catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis. The linkage of the epithelial E-cadherin/uvomorulin to actin is essential for the cell binding function of this cadherin. Catenins link E-cadherin to other integral membrane proteins such as Na+/K+ -ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, Src and Yes kinases and are modulated by Wnt-1 protooncogene. They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Within its conserved regions, alpha-catenin shows 30% idENTITY_TYPE_CODE to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions. Vinculin is known to interact with alpha-actinin, which in turn is associated with actin filaments in their site of attachment to the cell membrane focal contacts. alpha-Catenin appears to be capable of interacting with N-cadherin and P-cadherin. Absence of alpha-catenin is found in certain tumor cell lines. Frequent reduction of alpha-catenin levels in human carcinomas of the esophagus, stomach and colon is also reported.
Application: Rabbit polyclonal anti-alpha-Catenin antibody is used to tag alpha-catenin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as dot blot immunoassay and immunoblotting. Anti-alpha-Catenin may be used for the immuno-localization of alpha-catenin by various immunohistochemical methods using frozen tissue sections and cultured cells. It is used as a probe to determine the presence and roles of alpha-catenin in mediating the transduction of cell-cell contact positional signals to the cell interior.
Immunogen: synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse alpha-catenin conjugated to KLH.
Specificity: Does not cross-react with beta-catenin or gamma-catenin (plakoglobin).
RIDADR: NONH for all modes of transport
WGK Germany: nwg