alpha-Amylase from human saliva, Type XIII-A, lyophilized powder, 300-1,500 units/mg protein

Synonyms: 1,4-alpha-D-Glucan-glucanohydrolase

CAS Number: 9000-90-2

MDL No.: MFCD00081319

Storage: -20C

Enzyme Commission (EC) Number: 3.2.1.1  ( BRENDA | IUBMB )

EC Number: 232-565-6

UNSPSC Code: 12352204

General description: Human α-amylase is made up of 496 amino acids in a single polypeptide chain, which is encrypted on chromosome 1. These are produced either in the salivary glands or the pancreas. The salivary and pancreatic α-amylases have homologous primary sequence but exhibit different cleavage patterns.

General description: Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application: α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A1031 is from human saliva, is type IIA, and is supplied as a lyophilized powder. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis . α-Amylase from human saliva has been used to study the development of nutraceuticals, which may aid the treatment of diabetes and obesity .

Biochem/physiol Actions: α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Physical form: Lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride.

Unit Definition: One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 C.

Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer