Adenosine 5'-(beta,gamma-imido)triphosphate lithium salt hydrate, >=93% (HPLC), powder

Synonyms: beta,gamma-Imidoadenosine 5'-triphosphate lithium salt hydrate; AMP-PNP; ATP[beta,gamma-NH]; Adenylyl imidodiphosphate lithium salt hydrate; App(NH)p

CAS Number: 25612-73-1 (free acid)

Formula: C10H17N6O12P3 · xLi+ · yH2O

Formula Weight: 506.20 (free acid basis)

Linear Formula: C10H17N6O12P3 · xLi+ · yH2O

Purity: >=93% (HPLC)

Storage: -20C

Beilstein Registry Number: 6047109

UNSPSC Code: 41106305

Application: Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has been used in a study to research the effects of monovalent cations Li+, Na+, K+, NH4+, Rb+ and Cs+ on the solid and solution structures of the nucleic acid components. Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has also been used in a study to determine that antidepressants inhibit interferon-γ-induced microglial production of IL-6 and nitric oxide.

Biochem/physiol Actions: A non-hydrolyzable ATP analog. AMP-PNP competitively inhibits ATP-dependent enzyme systems, such as glutamine synthetase. It inhibits a number of enzymes which require the hydrolysis of ATP such as DNA topoisomerase II, SV40 large T-antigen helicase and a number of kinases. Blocks ATP-sensitive calcium-dependent potassium channels.

Reconstitution: A 10 mg/mL stock solution may be made, aliquoted and stored at -70 C for up to 3 months.