Synonyms: Aminoacylase; N-Acylamino acid amidohydrolase
CAS Number: 9012-37-7
MDL No.: MFCD00081285
Storage: -20C
Enzyme Commission (EC) Number: 3.5.1.14 ( BRENDA | IUBMB )
EC Number: 232-732-3
UNSPSC Code: 12352204
Application: Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .
Biochem/physiol Actions: Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .
Unit Definition: One unit will hydrolyze 1.0 mumole of N-acetyl-L-methionine per hr at pH 7.0 at 25 C.
Analysis Note: Protein determined by biuret.
RTECS: BF4890000
RIDADR: NONH for all modes of transport
WGK Germany: 1
R Codes: 36/37/38-42
S Codes: 22-24-26-36/37
IOD Codes: Xn
Symbol: GHS07, GHS08
Signal Word: Danger
Hazard Statements: H315-H319-H334-H335
Precautionary statements: P261-P305 + P351 + P338-P342 + P311