MDL No.: MFCD01092095
Storage: -20C
UNSPSC Code: 12352200
Features and Benefits: . Increased fidelity, up to 6.5x that of Taq DNA polymerase. Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.
Application: AccuTaq(TM) LA DNA Polymerase is utilized to amplify DNA sequences including genomic targets larger than 20 kb, as a result of a mixture of high quality Taq polymerase with a proofreading polymerase.Produce long DNA amplicons for:. Mutation analysis. Cloning genes. DNA sequencing. cDNA library generation
Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74C.
Other Notes: AccuTaq LA DNA polymerase is an optimized blend of Sigma's high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3'->5' exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Other Notes: Store AccuTaq LA DNA Polymerase and the 10x Buffer at -20Â C. Store DMSO at room temperature. Melting frozen DMSO at ~30Â C will not affect performance.
RIDADR: NONH for all modes of transport
Flash Point(F): 188.6 F
Flash Point(C): 87 C