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MilliporeSigma

SIGMA 4',6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, Greater Than or Equal to 98% (HPLC and TLC), suitable for fluorescence

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SIGMA 4',6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, Greater Than or Equal to 98% (HPLC and TLC), suitable for fluorescence

Synonym(s): 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

Formula: C16H15N5 · 2HCl

Formula Weight: 350.25

CAS No.: 28718-90-3

Purity: ≥98% (HPLC and TLC)

Application: DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Caution: Protect from light.

Storage: 2-8°C

Beilstein Registry Number: 4894417

EC No.: 249-186-7

Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Empirical Formula (Hill Notation): C9H10O

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Product Detail
Thomas No.
C980R42
Mfr. No.
D8417-1MG
Description
4',6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, Greater Than or Equal to 98% (HPLC and TLC), suitable for fluorescence, 1 mg
CAS Number
28718-90-3
Packaging Size
1 mg
list price/quantitytotal
$0.00
Thomas No.
C980R43
Mfr. No.
D8417-5MG
Description
4',6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, Greater Than or Equal to 98% (HPLC and TLC), suitable for fluorescence, 5 mg
CAS Number
28718-90-3
Packaging Size
5 mg
list price/quantitytotal
$0.00
Thomas No.
C980R44
Mfr. No.
D8417-10MG
Description
4',6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, Greater Than or Equal to 98% (HPLC and TLC), suitable for fluorescence, 10 mg
CAS Number
28718-90-3
Packaging Size
10 mg
list price/quantitytotal
$0.00
$0.00 (0 Items)
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