3-Phosphoglyceric Phosphokinase from baker's yeast (S. cerevisiae), ammonium sulfate suspension, >=1000 units/mg protein

Synonyms: 3-Phosphoglycerate kinase; ATP:3-Phospho-D-glycerate 1-phosphotransferase

CAS Number: 9001-83-6

MDL No.: MFCD00130301

Storage: 2-8C

Enzyme Commission (EC) Number: 2.7.2.3  ( BRENDA | IUBMB )

EC Number: 232-636-1

UNSPSC Code: 12352204

Application: 3-Phosphoglyceric Phosphokinase generates ATP by catalyzing the transfer of a phosphate group from 1,3-diphosphoglycerate to ADP. 3-Phosphoglycerate Phosphokinase is used to study glycolysis and gluconeogenesis. It has also been used to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase.

Application: The enzyme has been used in the assay of glyceraldehyde-3-phosphate dehydrogenase.

Biochem/physiol Actions: 3-Phosphoglyceric Phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate Phosphokinase activity is essential for glycolysis and gluconeogenesis.

Physical form: Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0

Unit Definition: One unit will convert 1.0 mumole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 C.

Analysis Note: Protein determined by Modified Warburg-Christian.