SDIP/Europium for membrane fusion assay

Biotium developed SDIP/Eu³ as an alternative to DPA/Tb³ , which is used for vesicle fusion assays. Similar to DPA/TB ³ assay, one population of vesicles can be loaded with the chelator SDIP while another population of vesicles can be loaded with EuCl₃. Intense red fluorescence is produced when the two types of vesicles fuse as a result of formation of the SDIP/Eu³ complex. The main advantage of SDIP/Eu³ over DPA/TB³ is that the SDIP/Eu³ generates fluorescent emission with brighter intensity and much longer wavelength. Each set of products contain 10 mg SDIP and 25 mg EuCl₃ in two separate vials. 

 
• &lamba;Abs/&lamba;Em(for complex) = 250 ~320/~610 nm 
• SDIP is a light yellow solid 
• EuCl³ is in a colorless crystal 
• Both are readily soluble in water 
• Both components are stable at room temperature or 4°C 
• Aqueous solutions of SDIP should be protected from light 
• MW of SDIP: 449 
• MW of EuCl³: ~258