Single dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations.
Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Drop Out Base (DOB) or Drop Out Base with 17 g Agar (DOBA) formula, with SC-His. DOB contains 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, and 20g Dextrose per liter. Chosen reagents are combined in the proper proportions according to the formulation listed on the Certificate of Analysis and milled to thoroughly blend the reagents for a uniform distribution. A sample of the milled and blended formulation was used to prepare liquid (or plate) medium by adding 2.7g of DOB (or 4.37g DOBA) powder and 0.192 g of SC-His to a 100mL of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 15 minutes. After cooling to 50°C, plates of DOBA with SC-His were poured.