Saponin is "built" from quillaic acid, a sugar and possibly other substances. It is a Glycoside widely distributed in plants.
1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.
Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes.
Grade: Practical Grade
Key Applications: Hemolyze erythrocytes, Permeabilize cultured human intestinal epithelial cells and paraformaldehyde-saponin- fixed human fibroblasts, Surface-active agent
Application Areas: Cell biology
Product Type: Biochemicals
Biochemical Category: Surfactants
Chemical Class: Surfactants
Presentation: White Powder
Format: Powder
pH: 3 - 7 (5% aqueous solution)
Detection Method: FTIR
Loss on Drying: ≤10%
Solubility: Freely soluble in alcohols. Soluble in water.
Storage & Handling: Store at Room Temperature (15-30°C).