Purity: >95% (SDS-PAGE)
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Biochem/physiol Actions:
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.
General description:
Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Preparation Note:
Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Sequence:
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.
Specificity:
Species specificity:Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.