ROCHE Transcriptor Reverse Transcriptase

UNSPSC Code: 12352200

RIDADR: NONH for all modes of transport

Features and Benefits:
• Achieve high sensitivity in two-step RT-PCR.
Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler^®Instruments).
• Obtain more full-length transcripts - up to 14 kb.
cDNA libraries with large inserts can be generated.
• Reverse transcribe difficult templates.
The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.
• Efficiently label cDNA.
Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.

Application:
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler^® Instruments) for the following applications:

• Synthesis of first-strand cDNA for use in subsequent amplification reactions
• RT-PCR of GC-rich RNA templates
• Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
• Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
• Generation of cDNA libraries with large inserts
• Dideoxy DNA sequencing
• RNA sequencing
• 3′-end labeling of DNA fragments
• Generation of single-stranded probes for genomic footprints

General description:

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Each lot of Transcriptor Reverse Transcriptase is routinely function tested in RT-PCR:

• with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
• with the LightCycler^® Instrument to detect 5 x 10² to 5 x 10⁶ copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.

Components:
• Transcriptor Reverse Transcriptase, in storage buffer
• Transcriptor RT buffer, 5x concentrated

Preparation Note:
Volume activity: 20 U/μl.
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:

• long targets, because it can transcribe up to 14 kb RNA templates
• rare targets, because it has high sensitivity
• GC-rich targets, because it can operate at high temperatures (up to +65^oC) to eliminate problems associated with extensive secondary structure

Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.

Legal Information:
LightCycler is a registered trademark of Roche