Synonyms: gene 32 protein, t4
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:
• Optimization of PCR (See "Product Description" below.)
• Stimulation of in vitro DNA synthesis
• Stabilization of single-stranded regions of DNA or RNA
• Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
• Helping restriction enzyme digestions go to completion
• q-PCR
General description:
T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 μg/100 μl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Absence of contaminants
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:
• nonspecific exo- and endonucleases
• single-strand DNases (nicking activities)
• single-strand-specific exonucleases
• RNases
Physical form:
Protein solution, 1 - 10mg/ml, in storage buffer (20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 0.5mM DTT, 50% glycerol [v/v], pH approximately 8.0)
Preparation Note:
Working concentration: 1 to 10 mg/ml
Specificity:
T4 gene 32 protein is a single-strand specific, helix-destabilizing protein encoded by gene 32 of the phage T4 genome. It is required for the replication of DNA and genetic recombination in Escherichia coli cells that are infected with T4 bacteriophage.