Synonyms: dna ligase, t4
Specific Activity: ~3000 units/mg protein
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Ligation of sticky- and blunt-ended DNA fragments.
General description:
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).
Specificity:
Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).